Wednesday 1 August 2018

Latest Research in Artificial Insemination


Research -1

Farmers’ Perceptions of Socio - Economic Biosafety Considerations Regarding Adoption and Use of Biotechnology: A Case Study of Sithobeleni and Lesibovu Communities in Swaziland
Agriculture plays a great role in the economy of Swaziland. It is also important for food production. Over 70% of the agriculture is practiced in the rural sector where mainly food crops are produced under subsistence farming systems characterized by large hectares cultivated with corn and a high cattle population producing low yields. Biotechnology has potential to improve agricultural production. Biosafety procedures have been developed to ensure safe handling of products of modern biotechnology. The aim of this study was to determine socio economic considerations for risk assessment when products of modern biotechnology are introduced into the environment. Information was obtained from key informants and focus discussion groups of cotton, maize and cattle farmers from Lesibovu and Sithobeleni communities. Key biotechnologies already practiced in the communities included production and use F1 hybrid maize seeds as well as artificial insemination in dairy cattle. The major socio economic factors that should be considered when new technologies are introduced include consideration of community norms and values such as seed sharing and seed saving, considerations of cultural and religious beliefs such as objection to eating of some animal species, and acceptability of products of modern biotechnology by markets. Major constraints limiting implementation of technologies in the communities include difficulties with acquisition of inputs, drought and shortage of advisory services. It was observed that farmers were willing to adopt modern biotechnology provided that it was safe to public health, animal health and the environment. The technology should be legally acceptable in the country and be approved for use elsewhere.  It was concluded that there is need to ensure that socio-economic factors form part of the risk assessment protocol when applications are considered for the release of products of modern biotechnology to the environment. Socio economic considerations should also form part of public awareness campaigns and training prior to the introduction of modern biotechnology into communities.


Research -2

Studies on the Effect of Lugol’s Iodine on Reproductive Efficiency of Dairy Cattle
The present investigation was conducted to study the effect of intra uterine infusion of 20 ml Lugol’s iodine (0.25 %) on day 5 (Group I; n= 18), day 17 (Group II n= 17) on reproductive performance as compared to untreated animals (Group III; n= 18). The average inter estrus interval in the animals in treatment group I (14.12 ± 0.396 days) was significantly (P<0.01) shorter than group II (23.93 ± 0.330 days) and control group III (20.93 ± 0.870 days). The average duration (days) for first postpartum fertile estrus was shortest (88.93 ± 10.053 days) in group I, followed by group II (106.46 ± 8.743 days) and control group III (113.72 ± 7.131 days). The number of artificial insemination (A.I.) required per conception was observed to be only 1.38 in group I while the values were 1.50 and 1.91 for group II and III, respectively.


Research -3

Effect of Skim Milk and Tris-citrate Extenders to Preserve the Semen of Indigenous Ram of Bangladesh
In recent years, there has been an increased interest in the sperm preservation and maintenance of genetic resources of small ruminants. The study aimed to determine and compare the preservation effects of skim milk and Tris-citrate on the quality of chilled and frozen-thawed indigenous ram semen (n=13) during rainy monsoon season (June to October) in Bangladesh. Semen was collected from four indigenous rams by artificial vagina method to observe motility, viability and membrane integrity at 0, 24, 48, 72 hrs of storage. The hypo-osmotic resistance test (HOST) was used to evaluate the sperm membrane integrity. The ejaculates were divided into two groups according to dilution with skim milk or Tris-citric acid egg yolk media for chilling and freezing. Motility, viability and membrane integrity were significantly (p<0.01) higher in chilled semen samples diluted with skim milk in comparison to those of semen diluted with Tris-based extender at all time-based observations. However, around 50% motility was preserved up to 48 hours by both extenders. Results also showed that motility, viability and HOST (+ve %) decreased drastically after cryopreservation irrespective of diluents. Similar to chilled semen, studied parameters were significantly higher (p<0.05) in frozen-thawed semen diluted with skim milk based extender, though quality parameter of frozen-thawed semen was not satisfactory. In conclusion, skim milk based extender appears to be a better option compared to Tris based extenders for chilling semen of indigenous ram up to 48 hours with acceptable quality. Whether the differences found between the extenders will be reflected in the fertility results after artificial insemination (AI) is yet unknown and needs to be further studied.

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